Directional control of the formation rate, and structural and mechanical properties of yuba films via disulfide bond- or ionic interaction-mediated soy protein aggregation.
Study Goal
The researchers aimed to determine whether introducing calcium ions and sodium metabisulfite (SM) could enhance the formation rate and tensile strength of yuba films made from soy.
Results Summary
The study found that calcium ions increased the density and continuity of yuba films, while SM led to larger protein particle formation, improving film properties. The size of protein particles increased with SM concentration, and basic polypeptides from glycinin were released during SM treatment, contributing to structural improvements.
Population
Not specified (laboratory study on soy protein films)
Effective Dosage
SM concentrations ranged from 0 to 20 mmol L-1
Duration
Not specified
Interactions
None mentioned
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
introducing calcium ions | increase | formation rate and tensile strength of yuba films | yuba films | - | enhanced | #1 |
introducing sodium metabisulfite (SM) | increase | formation rate and tensile strength of yuba films | yuba films | - | enhanced | #2 |
calcium ion | increase | density and continuity of the yuba films | yuba films | - | increased | #3 |
sodium metabisulfite (SM) | increase | larger protein particles | yuba films | - | led to the formation of | #4 |
concentration of SM | increase | size of the largest protein particles | yuba films | from 112.8 to 408.6 nm | rose | #5 |
SM treatment | increase | basic polypeptides from glycinin (11S) | yuba films | - | release of | #6 |
heating | neutral | exposed hydrophobic residues of the basic polypeptides | protein particles | - | became trapped inside | #7 |
structure and functional properties of protein particles | neutral | formation rate and tensile strength of yuba films | yuba films | - | significantly impacted | #8 |
BACKGROUND: Yuba is a worldwide popular soybean product, but its development is currently challenged by the time-consuming fabrication process. RESULTS: In this study, we proposed a simple yet effective strategy to enhance the formation rate and tensile strength of yuba films by introducing calcium ions and sodium metabisulfite (SM). Results show that calcium ion increased the density and continuity of the yuba films, while SM led to the formation of larger protein particles. Moreover, dynamic light scattering indicated that the size of the largest protein particles increased from 112.8 to 408.6 nm as the concentration of SM rose from 0 to 20 mmol L-1. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis results revealed a strong correlation between the formation of large protein particles and the release of basic polypeptides from glycinin (11S) during SM treatment. Furthermore, the blue shift of the maximum emission wavelengths of fluorescence intensity suggested that the exposed hydrophobic residues of the basic polypeptides became trapped inside the protein particles upon heating. CONCLUSION: The results demonstrated that the structure and functional properties of protein particles significantly impacted the formation rate and tensile strength of yuba films. These findings offer valuable and fundamental insights for the advancement of industrial production of yuba films. © 2025 Society of Chemical Industry.