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A Pilot 24-Week 'Bulk and Cut' Dietary Protocol Combined with Resistance Training Is Feasible and Improves Body Composition and TNF-α Concentrations in Untrained Adult Males.

Nutrients
April 4, 2025
Anthony J Giannopoulos et al. (7 authors)
Journal ArticleHuman Study
Study Details

Study Goal

The researchers aimed to evaluate the effects of a 24-week bodybuilding program combining resistance training with a dietary bulk-and-cut protocol on body composition, muscle strength, and inflammatory markers in middle-aged males with obesity.

Results Summary

The intervention significantly increased muscle strength (deadlift +46%, squat +65%) and improved body composition (fat-free mass +3%, body fat -6%). It also reduced resting TNF-α concentrations by 15%, indicating reduced inflammation, though other inflammatory markers and lipids did not change significantly.

Population

Middle-aged untrained males with obesity (BMI = 35.0 ± 4.6 kg/m², body fat = 36 ± 5%).

Effective Dosage

Not specified (resistance training combined with 12-week cycles of caloric bulking and cutting).

Duration

24 weeks.

Interactions

None mentioned.

Extracted Claims (12)
InterventionDirectionEndpointPopulationDosageImpactClaim #
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
increase
muscle strength
middle-aged adult males with obesity
-
led to significant increases
#1
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
increase
deadlift strength
middle-aged adult males with obesity
+46%
significant increases
#2
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
increase
squat strength
middle-aged adult males with obesity
+65%
significant increases
#3
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
increase
fat-free mass
middle-aged adult males with obesity
+3%
Improvements in body composition were characterized by an increase
#4
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
decrease
body fat percentage
middle-aged adult males with obesity
-6%
Improvements in body composition were characterized by a decrease
#5
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
no change
circulating lipids (triglycerides, total, low-density, and high-density cholesterol)
middle-aged adult males with obesity
no significant change
did not change significantly
#6
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
no change
C-reactive protein (CRP)
middle-aged adult males with obesity
no significant change
did not change significantly
#7
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
no change
interleukin-6 (IL-6)
middle-aged adult males with obesity
no significant change
did not change significantly
#8
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
no change
interleukin-10 (IL-10)
middle-aged adult males with obesity
no significant change
did not change significantly
#9
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
decrease
tumor necrosis factor-alpha (TNF-α)
middle-aged adult males with obesity
15% lower concentrations at week 24 compared to baseline
notable reduction
#10
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
increase
body composition
middle-aged adult males with obesity
-
leading to improvements
#11
24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol
decrease
resting TNF-α concentrations
middle-aged adult males with obesity
-
lower
#12
Abstract

Background/Objectives: This study piloted a 24-week bodybuilding program combining resistance training (RT) with a dietary bulk-and-cut protocol in middle-aged adult males. Methods: Seven untrained males (33 ± 3.0 years; BMI = 35.0 ± 4.6 kg/m2; body fat = 36 ± 5%) completed a 24-week intervention combining RT with a dietary protocol consisting of 12-week cycles of caloric bulking (0-12 weeks) and cutting (12-24 weeks). The participant retention rate was 64%, while compliance with training was 96.7%, and adherence to dietary cycles was over 93%. To assess the preliminary efficacy of the intervention, venous blood samples and measurements of body composition (BodPod), muscle strength, and VO2max (cycle ergometer) were collected at baseline (week 0) and following the bulking (week 12) and cutting (week 24) cycles. Circulating lipids (triglycerides, total, low-density, and high-density cholesterol), C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) were measured in serum. Results: The training led to significant increases in muscle strength, especially in the deadlift (+46%, p < 0.001) and squat (+65%, p < 0.001). Improvements in body composition were characterized by an increase in fat-free mass and a decrease in body fat percentage over the 24-week intervention (+3% and -6%, respectively, p < 0.05). Lipids, CRP, IL-6, and IL-10 did not change significantly, but there was a notable reduction in TNF-α (time effect p = 0.05, pη2 = 0.39), with 15% lower concentrations at week 24 compared to baseline, indicating reduced inflammation. Conclusions: Overall, the pilot intervention achieved high compliance and adherence rates, leading to improvements in body composition and lower resting TNF-α concentrations in a group of middle-aged males with obesity.

Medical Subject Headings (MeSH)
HumansMaleResistance TrainingBody CompositionTumor Necrosis Factor-alphaAdultPilot ProjectsMuscle StrengthDietC-Reactive ProteinInterleukin-6Feasibility StudiesInterleukin-10
Study Links
Quality Scores
Safety85
Efficacy80/10
Quality70/10
Research Impact Scores
APT Score0.05
Weight Score1.20
Normalized Score0.80
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