Deleterious impacts of Western diet on jejunum function and health are reversible.
Study Goal
The researchers aimed to determine whether the negative effects of a high-fat high-sugar (Western) diet on intestinal function and health were reversible.
Results Summary
The study found that the Western diet significantly reduced basal jejunal short circuit current (Isc), attributed to decreased ClC2 expression, but this effect was fully reversed upon switching to a standard diet. Changes in inflammatory markers and intestinal health were linked to body weight fluctuations.
Population
Male C57Bl/6J mice
Effective Dosage
Not specified
Duration
24 weeks (12 weeks for the group switched to standard diet)
Interactions
None mentioned
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
high-fat high-sugar diet (Western diet) for 24 wk | decrease | Basal transepithelial short circuit current (Isc) | male C57Bl/6J mice | by 42% | significantly decreased | #1 |
switching to standard chow and water for 12 wk after HFHS diet | increase | decrease in basal Isc | male C57Bl/6J mice | - | fully reversed | #2 |
high-fat high-sugar diet (Western diet) | decrease | calcium-activated chloride channel (ClC2) expression | male C57Bl/6J mice | - | significant loss | #3 |
high-fat high-sugar diet (Western diet) | neutral | inflammatory state (TNF-α) | male C57Bl/6J mice | - | associated with | #4 |
high-fat high-sugar diet (Western diet) | neutral | intestinal health [myeloperoxidase (MPO) activity] | male C57Bl/6J mice | - | associated with | #5 |
high-fat high-sugar diet (Western diet) | neutral | body weight changes | male C57Bl/6J mice | - | associated with | #6 |
The goal of this study was to determine whether the influence of a high-fat high-sugar diet (Western diet) on intestinal function and health was reversible. We measured transepithelial short circuit current (Isc), across freshly isolated segments of jejunum from male C57Bl/6J mice randomly assigned to one of the following groups for the study duration: high-fat high-sugar diet for 24 wk (HFHS), HFHS diet for 12 wk then switched to standard chow and water for a further 12 wk (Std), and lean controls (standard chow and water for 24 wk). At the completion of the study, segments of jejunum were frozen for Western blot determination of key proteins involved in secretory and absorptive functions, as well as senescence. Intestinal morphology was assessed. Serum and tissue assays were performed. Basal Isc was significantly decreased (by 42%, P < 0.05) in HFHS versus leans. This decrease in Isc was fully reversed by switching to Std diet. The HFHS-induced decrease in Isc was attributed to a significant loss of calcium-activated chloride channel (ClC2) expression. Changes in inflammatory state (TNF-α) and intestinal health [myeloperoxidase (MPO) activity] were associated with body weight changes. Our data suggests that the reduced basal jejunal Isc in HFHS mice is reversible. Better understanding of Western diet-mediated intestinal disturbances may permit for improved treatment options for gastrointestinal abnormalities in obese individuals.NEW & NOTEWORTHY Our data suggests that the reduced basal jejunal Isc (decreased secretory function) in Western diet-fed mice is reversible. A better understanding of Western diet-mediated intestinal disturbances may permit improved treatment options for gastrointestinal abnormalities in obese individuals.