Exploring the promising potential of alcohol extract from the aerial part of dill in ameliorating DSS-induced ulcerative colitis in mice.
Study Goal
The researchers aimed to investigate the potential of dill alcohol extract (DE) in ameliorating ulcerative colitis (UC) induced by Dextran Sulfate Sodium Salt (DSS) in mice, focusing on anti-inflammatory, oxidative stress reduction, and intestinal barrier protection effects.
Results Summary
DE significantly reduced disease activity and colon damage in UC mice, alleviated oxidative stress and inflammation, protected the intestinal barrier, and improved gut flora balance. The study did not report major limitations but was conducted in mice, necessitating further human trials.
Population
C57BL/6 mice with DSS-induced ulcerative colitis.
Effective Dosage
200 mg/kg (low dose) and 400 mg/kg (high dose) administered daily by gavage.
Duration
7 days of intervention.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
alcohol extract from the aerial part of dill (DE) | decrease | disease activity index (DAI) | DSS-induced UC mice | - | significantly reduces | #1 |
alcohol extract from the aerial part of dill (DE) | decrease | colon histopathological damage | DSS-induced UC mice | - | significantly reduces | #2 |
alcohol extract from the aerial part of dill (DE) | decrease | IL-6 levels | UC mice | - | reducing | #3 |
alcohol extract from the aerial part of dill (DE) | decrease | IL-1β levels | UC mice | - | reducing | #4 |
alcohol extract from the aerial part of dill (DE) | decrease | MDA levels | UC mice | - | reducing | #5 |
alcohol extract from the aerial part of dill (DE) | decrease | MPO levels | UC mice | - | reducing | #6 |
alcohol extract from the aerial part of dill (DE) | increase | CAT levels | UC mice | - | increasing | #7 |
alcohol extract from the aerial part of dill (DE) | increase | GSH levels | UC mice | - | increasing | #8 |
alcohol extract from the aerial part of dill (DE) | decrease | goblet cells | UC mice | - | reducing damage to | #9 |
alcohol extract from the aerial part of dill (DE) | increase | mucin MUC2 | UC mice | - | increasing the levels of | #10 |
alcohol extract from the aerial part of dill (DE) | neutral | tight junction proteins (ZO-1, Occludin, Claudin-1, Claudin-2) | UC mice | - | regulating the expression of | #11 |
alcohol extract from the aerial part of dill (DE) | increase | ratio of beneficial and harmful bacteria | UC mice | - | improves | #12 |
alcohol extract from the aerial part of dill (DE) | decrease | imbalance of intestinal flora | UC mice | - | alleviating | #13 |
alcohol extract from the aerial part of dill (DE) | decrease | inflammation | LPS-induced RAW264.7 cells | - | has anti-inflammatory activity | #14 |
ETHNOPHARMACOLOGICAL RELEVANCE: Dill (Anethum graveolens L.) is a typical Uyghur medicine. It is traditionally used to treat sticky and stagnant dampness, hiccups and food stagnation, intestinal obstruction, and anorectal diseases. STUDY OBJECTIVE: Our study is designed to investigate the potential of alcohol extract from the aerial part of dill in ameliorating ulcerative colitis induced by Dextran Sulfate Sodium Salt (DSS) in mice. MATERIALS AND METHODS: In this paper, the chemical composition of the aerial part of dill was speculated from the data obtained by LC-MS and determined by comparing with 10 standards through HPLC. The aerial part of fresh dill was dried, crushed, sieved, and then extracted with 70% ethanol to obtain DE. The lipopolysaccharide (LPS)-induced RAW264.7 cells were used to test the anti-inflammatory activity of DE in vitro. The impact of DE on UC was also studied in vivo. UC was induced by drinking 2.5% DSS to C57BL/6 mice for 6 days. The positive control group received 5-aminosalicylic acid (5-ASA) by gavage, and the low and high-dose treatment groups were respectively given 200 mg/kg and 400 mg/kg of DE by gavage daily for 7 days from the first day. RESULTS: DE significantly reduces the disease activity index (DAI) and colon histopathological damage. DE can also alleviate oxidative stress and inflammation in UC mice by reducing IL-6, IL-1β, MDA, and MPO levels and increasing CAT and GSH levels in colonic tissues. DE can protect the integrity of the colonic mucosal barrier by reducing damage to goblet cells, increasing the levels of mucin MUC2, and regulating the expression of tight junction proteins such as ZO-1, Occludin, Claudin-1, and Claudin-2. In addition, DE improves the ratio of beneficial and harmful bacteria, thus further alleviating the imbalance of intestinal flora. CONCLUSION: DE has anti-inflammatory activity in vitro and an ameliorative effect on DSS-induced UC in mice by alleviating oxidative stress and inflammation, protecting the integrity of the intestinal barrier, and regulating intestinal flora.