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Vitamin C-Rich Guava Consumed with Mungbean Dal Reduces Anemia and Increases Hemoglobin but not Iron Stores: A Randomized Controlled Trial of Food-to-Food Fortification in Indian Children.

The Journal of nutrition
December 1, 2024
Varsha Rani et al. (7 authors)
Journal ArticleRandomized Controlled TrialHuman StudyClinical
Study Details

Study Goal

The researchers aimed to assess whether adding vitamin C-rich guava to an iron-containing mungbean meal improved iron bioavailability, hemoglobin levels, and anemia in children.

Results Summary

Adding guava to the meal increased hemoglobin levels and reduced anemia prevalence but did not significantly improve body iron stores. The effects were more pronounced in iron-deficient children.

Population

6- to 10-year-old school children (n = 200; 46% anemic, 71% iron-deficient) from a rural community in Haryana, North India.

Effective Dosage

~170 mg vitamin C daily (via guava) with 3.2 mg iron.

Duration

7 months

Interactions

None mentioned

Extracted Claims (7)
InterventionDirectionEndpointPopulationDosageImpactClaim #
mungbean dal with fresh guava
no change
body iron stores
6- to 10-y-old school children from a rural community in Haryana, North India
mean treatment effect: 0.65 mg/kg body weight; 95% confidence interval (CI): -0.34, 1.63; P = 0.197
did not result in an overall improvement
#1
mungbean dal with fresh guava
increase
hemoglobin concentration
children in the guava group
3.7 g/L; 95% CI: 1.6, 5.6; P = 0.001
showed a larger increase
#2
mungbean dal with fresh guava
decrease
prevalence of anemia
children in the guava group
-51%; 95% CIs: -74, -10; P = 0.022
showed a larger drop
#3
mungbean dal with fresh guava
decrease
prevalence of iron-deficiency anemia
children in the guava group
-56%, 95% CI: -83, 13; P = 0.087
showed a larger drop
#4
Addition of guava to a mungbean-based meal containing a moderate amount of iron
increase
hemoglobin
children
-
increased
#5
Addition of guava to a mungbean-based meal containing a moderate amount of iron
decrease
anemia
children
-
reduced
#6
Addition of guava to a mungbean-based meal containing a moderate amount of iron
no change
body iron stores
children
-
did not provide enough additional absorbed iron to also increase
#7
Abstract

BACKGROUND: Adding vitamin C-rich fruit to staples containing iron could be an effective strategy to improve iron bioavailability and thereby reduce iron-deficiency anemia in children. OBJECTIVES: We aimed to assess the effect of consuming a mungbean-based meal with or without guava fruit on body iron stores, hemoglobin concentration, and anemia of children as part of a school feeding program. METHODS: We conducted a 7-mo randomized, controlled trial with 6- to 10-y-old school children (n = 200; 46% anemic, 71% iron-deficient) from a rural community in Haryana, North India. Children were assigned to 2 treatment groups to daily receive either a meal of mungbean dal only (3.0 mg iron; vitamin C:iron molar ratio ∼0.5:1), or mungbean dal with fresh guava (3.2 mg iron; ∼170 mg vitamin C; molar ratio ∼18:1). Meals were served every school day under supervision. The primary outcome was body iron stores, whereas concentrations of hemoglobin and other iron indicators were secondary outcomes. RESULTS: Daily consumption of mungbean dal along with guava did not result in an overall improvement of body iron stores [mean treatment effect: 0.65 mg/kg body weight; 95% confidence interval (CI): -0.34, 1.63; P = 0.197]. However, compared with children who consumed mungbean dal only, children in the guava group showed a larger increase in hemoglobin concentration (3.7 g/L; 95% CI: 1.6, 5.6; P = 0.001), and a larger drop in the prevalence of anemia (-51%; 95% CIs: -74, -10; P = 0.022) and iron-deficiency anemia (-56%, 95% CI: -83, 13; P = 0.087). These effects were more pronounced in children who were iron deficient at study start. CONCLUSIONS: Addition of guava to a mungbean-based meal containing a moderate amount of iron increased hemoglobin and reduced anemia but did not provide enough additional absorbed iron to also increase body iron stores. Food-to-food fortification by inclusion of vitamin C-rich fruits in iron-containing school meals may help alleviate the burden of anemia in children. TRIAL REGISTRATION NUMBER: This trial was registered at https://clinicaltrials.gov/ct2/show/NCT01191463.

Medical Subject Headings (MeSH)
HumansAscorbic AcidPsidiumChildHemoglobinsFemaleAnemia, Iron-DeficiencyMaleIndiaFood, FortifiedVignaIronFruit
Study Links
Quality Scores
SafetyNot Assessed
Efficacy70/10
Quality85/10
Research Impact Scores
APT Score0.05
Weight Score2.53
Normalized Score0.65
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