Fish Oil Supplementation Mitigates High-Fat Diet-Induced Obesity: Exploring Epigenetic Modulation and Genes Associated with Adipose Tissue Dysfunction in Mice.
Study Goal
The researchers aimed to determine whether fish oil (enriched with EPA) could mitigate high-fat diet-induced obesity in mice, focusing on epigenetic modifications in white adipose tissue and the role of adipose-derived stem cells.
Results Summary
Fish oil supplementation attenuated high-fat diet-induced obesity, reduced fat accumulation, and partially reversed epigenetic changes in white adipose tissue. The study also suggested a potential mechanism involving leptin signaling in adipose-derived stem cells, though it was conducted in mice, limiting direct human applicability.
Population
C57BL/6j mice fed a high-fat diet for 16 weeks.
Effective Dosage
Not specified
Duration
8 weeks of fish oil intervention (within a 16-week high-fat diet period).
Interactions
None mentioned
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
fish oil (FO) treatment, particularly enriched with eicosapentaenoic acid (EPA) | decrease | effects of HFD (increased body mass, fat accumulation, altered gene expression) | C57BL/6j mice | - | attenuated | #1 |
fish oil (FO) treatment, particularly enriched with eicosapentaenoic acid (EPA) | decrease | HFD-induced obesity | C57BL/6j mice | - | demonstrated efficacy in mitigating | #2 |
fish oil (FO) treatment, particularly enriched with eicosapentaenoic acid (EPA) | decrease | HFD-induced changes in histone H3K27 | C57BL/6j mice | - | partially reversed | #3 |
high-fat diet (HFD) | increase | obesity | C57BL/6j mice | - | induced | #4 |
high-fat diet (HFD) | increase | body mass | C57BL/6j mice | - | displayed increased | #5 |
high-fat diet (HFD) | increase | fat accumulation | C57BL/6j mice | - | displayed increased | #6 |
high-fat diet (HFD) | increase | gene expression associated with WAT inflammation and dysfunction | C57BL/6j mice | - | altered | #7 |
high-fat diet (HFD) | increase | histone H3K27 | C57BL/6j mice | - | induced changes in | #8 |
leptin | increase | ASC dysfunction | adipose-derived stem cells (ASCs) | - | suggesting a potential mechanism for | #9 |
This study investigated the effects of fish oil (FO) treatment, particularly enriched with eicosapentaenoic acid (EPA), on obesity induced by a high-fat diet (HFD) in mice. The investigation focused on elucidating the impact of FO on epigenetic modifications in white adipose tissue (WAT) and the involvement of adipose-derived stem cells (ASCs). C57BL/6j mice were divided into two groups: control diet and HFD for 16 weeks. In the last 8 weeks, the HFD group was subdivided into HFD and HFD + FO (treated with FO). WAT was removed for RNA and protein extraction, while ASCs were isolated, cultured, and treated with leptin. All samples were analyzed using functional genomics tools, including PCR-array, RT-PCR, and Western Blot assays. Mice receiving an HFD displayed increased body mass, fat accumulation, and altered gene expression associated with WAT inflammation and dysfunction. FO supplementation attenuated these effects, a potential protective role against HFD-induced obesity. Analysis of H3K27 revealed HFD-induced changes in histone, which were partially reversed by FO treatment. This study further explored leptin signaling in ASCs, suggesting a potential mechanism for ASC dysfunction in the obesity-rich leptin environment of WAT. Overall, FO supplementation demonstrated efficacy in mitigating HFD-induced obesity, influencing epigenetic and molecular pathways, and shedding light on the role of ASCs and leptin signaling in WAT dysfunction associated with obesity.