Effect of Garcinia binucao Crude Leaf Extract Supplementation on Lifespan of Drosophila melanogaster Chronically Exposed to Alcohol.
Study Goal
The researchers aimed to determine whether Garcinia binucao extract (GBE) supplementation could mitigate the lifespan reduction in Drosophila melanogaster caused by chronic alcohol exposure.
Results Summary
In flies without alcohol exposure, GBE supplementation did not improve lifespan compared to controls. However, in alcohol-exposed flies, high-dose GBE showed the longest mean lifespan, though differences were not statistically significant. GBE supplementation partially ameliorated the lifespan reduction caused by alcohol exposure.
Population
Drosophila melanogaster (fruit flies)
Effective Dosage
High dose (1 mg/mL), low dose (200 µg/mL)
Duration
Lifespan monitoring (duration not explicitly stated)
Interactions
None mentioned
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
Garcinia binucao extract (GBE) supplementation (high-dose, 1 mg/mL) | decrease | mean lifespan | Drosophila melanogaster without alcohol exposure | 34.42 days (vs control 38.15 days) | mean lifespan was | #1 |
Garcinia binucao extract (GBE) supplementation (low-dose, 200 µg/mL) | decrease | mean lifespan | Drosophila melanogaster without alcohol exposure | 33.24 days (vs control 38.15 days) | mean lifespan was | #2 |
DMSO | decrease | mean lifespan | Drosophila melanogaster without alcohol exposure | 22.29 days (vs control 38.15 days) | mean lifespan was | #3 |
Garcinia binucao extract (GBE) supplementation (high-dose, 1 mg/mL) | increase | mean lifespan | Drosophila melanogaster chronically exposed to alcohol | 33.80 days | the longest mean lifespan was observed | #4 |
Garcinia binucao extract (GBE) supplementation (low-dose, 200 µg/mL) | increase | mean lifespan | Drosophila melanogaster chronically exposed to alcohol | 33.63 days | mean lifespan was | #5 |
DMSO | increase | mean lifespan | Drosophila melanogaster chronically exposed to alcohol | 30.30 days | mean lifespan was | #6 |
control | increase | mean lifespan | Drosophila melanogaster chronically exposed to alcohol | 29.65 days | mean lifespan was | #7 |
chronic alcohol exposure | decrease | mean lifespan | Drosophila melanogaster | by 9.51 days | mean lifespan of the control group significantly decreased | #8 |
chronic alcohol exposure | decrease | mean lifespan | Drosophila melanogaster treated with high-dose GBE | by 0.82 days | mean lifespan significantly decreased | #9 |
chronic alcohol exposure | increase | mean lifespan | Drosophila melanogaster treated with low-dose GBE | by 0.39 days | mean lifespan significantly increased | #10 |
Garcinia binucao extract supplementation | increase | lifespan | Drosophila melanogaster chronically exposed to alcohol | - | ameliorated the observed reduction in lifespan | #11 |
BACKGROUND: Consumption and abuse of alcohol remains a significant cause of concern worldwide. Furthermore, there is evidence of the association between chronic alcohol use and reduced life expectancy. OBJECTIVES: To study the effects of Garcinia binucao extract (GBE) supplementation on lifespan of Drosophila melanogaster, in the presence or absence of chronic alcohol exposure. METHODS: D. melanogaster was mass cultured and given GBE supplementation in high (1 mg/mL) and low (200 µg/mL) sublethal doses. D. melanogaster flies were divided into groups - with and without chronic alcohol exposure, and their respective lifespans were monitored. RESULTS: In D. melanogaster without alcohol exposure, mean lifespan was highest in the control flies (38.15 days), followed by high-dose GBE (34.42 days), low-dose GBE (33.24 days), and DMSO (22.29 days). In D. melanogaster chronically exposed to alcohol, the longest mean lifespan was observed in flies treated with high-dose GBE (33.80 days), followed by low-dose GBE (33.63 days), the DMSO group (30.30), and the control group (29.65 days), but the differences were not statistically significant. Comparing groups with and without chronic alcohol exposure, the mean lifespan of the control group chronically exposed to alcohol significantly decreased by 9.51 days (p < 0.05). In GBE treatment groups, mean lifespan significantly decreased by 0.82 days in high-dose set-up (p < 0.05), and significantly increased by 0.39 days in the low-dose set-up (p < 0.05) upon chronic alcohol exposure. CONCLUSION: Garcinia binucao extract supplementation ameliorated the observed reduction in lifespan of Drosophila melanogaster chronically exposed to alcohol.