Randomized controlled trial of vitamin d supplementation on toll-like receptor-2 (tlr-2) and toll-like receptor-4 (tlr-4) in tuberculosis spondylitis patients.
Study Goal
The researchers aimed to investigate the effect of oral vitamin D supplementation on TLR-2 and TLR-4 levels in tuberculosis spondylitis patients.
Results Summary
Vitamin D supplementation at 10,000 IU and 5,000 IU doses significantly increased vitamin D, TLR-2, and TLR-4 levels by weeks 4 and 8, while the control group showed no significant increase. The study concluded that vitamin D supplementation can enhance TLR-2 and TLR-4 levels, with 10,000 IU/day for 8 weeks raising vitamin D levels sufficiently for optimal immunomodulatory effects.
Population
Tuberculosis spondylitis patients
Effective Dosage
10,000 IU/day and 5,000 IU/day
Duration
8 weeks
Interactions
None mentioned
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
oral supplementation with vitamin D | increase | levels of vitamin D | tuberculosis spondylitis patients | - | significant increases | #1 |
oral supplementation with vitamin D | increase | TLR-2 levels | tuberculosis spondylitis patients | - | significant increases | #2 |
oral supplementation with vitamin D | increase | TLR-4 levels | tuberculosis spondylitis patients | - | significant increases | #3 |
vitamin D supplements | increase | TLR-2 levels | - | - | can significantly increase | #4 |
vitamin D supplements | increase | TLR-4 levels | - | - | can significantly increase | #5 |
Supplementation with vitamin D 10,000 IU/day for 8 weeks | increase | vitamin D levels | - | > 50 ng/dl | can increase | #6 |
BACKGROUND: Tuberculosis spondylitis accounts for approximately 50% of all cases of skeletal tuberculosis. Vitamin D plays a role in the immune system. Vitamin D helps in the activation of TLR-2 and TLR-4, which play a role in the process of tuberculosis infection. The objective of this study was to investigate the effect of oral supplementation with vitamin D on TLR-2 and TLR-4 levels in tuberculosis spondylitis patients. METHODS: The true Experiment Design Pretest-Posttest with Control Group (Pretest-Posttest with Control Group) was used for this research. TLR-2 and TLR-4 were measured by ELISA. Repeated ANOVA, ANOVA tests, and Kolmogorov-Smirnov normality tests on the SPSS program were used to statistically analyze the results. RESULT: In the dose groups of 10,000 IU and 5000 IU, significant increases in the levels of vitamin D, TLR-2, and TLR-4 were observed at weeks 4 and 8 (p < 0.05). In the control group, there was no significant increase. CONCLUSIONS: Vitamin D supplements can significantly increase TLR-2 and TLR-4 levels. Supplementation with vitamin D 10,000 IU/day for 8 weeks can increase vitamin D levels > 50 ng/dl to optimally act as an immunomodulator.