Plantain-based diet decreases oxidative stress and inflammatory markers in the testes of rats exposed to atrazine.
Study Goal
The researchers aimed to determine whether a plantain-based diet (PBD) could protect against or reverse the oxidative and inflammatory damage caused by atrazine (ATZ) exposure in rat testes, focusing on male fertility.
Results Summary
The study found that PBD restored oxid-inflammatory homeostasis, reduced oxidative stress markers, and improved purinergic activity in ATZ-exposed rat testes. The diet contained 16 bioactive compounds, with quercetin being the most prominent, and showed both preventive and therapeutic efficacy. No significant limitations were noted, but the study was conducted in rats, not humans.
Population
Male rats exposed to atrazine (ATZ).
Effective Dosage
50%, 25%, and 12.5% PBD (preventive and therapeutic models).
Duration
Two-phased experiment (duration not explicitly stated).
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
atrazine (ATZ) exposure | decrease | male fertility | - | - | has deleterious effects | #1 |
atrazine (ATZ) treatment | increase | oxidative stress parameters (DNA fragmentation and MDA level) | rats | - | increased | #2 |
atrazine (ATZ) treatment | increase | purinergic activity (ATPase) | rats | - | increased | #3 |
atrazine (ATZ) treatment | increase | acetylcholine esterase | rats | - | increased | #4 |
atrazine (ATZ) treatment | increase | inflammatory markers (NO level, MPO activity, and TNF-α) | rats | - | increased | #5 |
atrazine (ATZ) treatment | decrease | Nrf2 levels | rats | - | decreased | #6 |
plantain-based diet (PBD) | neutral | oxido-inflammatory parameters | rat testes | - | was able to restore | #7 |
plantain-based diet (PBD) | neutral | toxicity caused by ATZ | rat testes | - | was able to protect and prevent | #8 |
Exposure to the herbicide atrazine (ATZ) has deleterious effects on male fertility. This fact underscores the need for measures to protect against the detrimental impact of atrazine exposure on male fertility. The study assessed the protective effects of plantain-based diet (PBD) on rat testes exposed to ATZ by exploring oxid-inflammatory homeostasis. The study evaluated the preventive and therapeutic effects of PBD in a two-phased experiment. Male rats were randomized into seven groups for therapeutic model (Control, ATZ only, ATZ recovery, ATZ + 50% PBD, ATZ + 25% PBD, ATZ + 12.5% PBD and ATZ + quercetin-QUE) while the preventive model had ten groups (Control, ATZ, 50% PBD + ATZ, 25% PBD + ATZ, 12.5% PBD + ATZ and QUE + ATZ). The oxidative stress parameters (DNA fragmentation and MDA level), purinergic activity (ATPase), acetylcholine esterase, and inflammatory markers (NO level, MPO activity, and TNF-α) were increased while the Nrf2 levels were decreased by the ATZ treatment. However, the PBD was able to restore the oxido-inflammatory parameters in the rat testes. The chemical fingerprint of the diet revealed that the diets contained 16 bioactive compounds with quercetin being the most prominent compound. Overall, treatment with PBD was able to protect and prevent the toxicity caused by ATZ by modulating the redox and inflammatory status as well as purinergic activity in the rat testes.