Evaluating neem gum-polyvinyl alcohol (NGP-PVA) blend nanofiber mat as a novel platform for wound healing in murine model.
Study Goal
The researchers aimed to evaluate the potential of Neem gum polysaccharide (NGP) combined with Polyvinyl alcohol (PVA) as a nanofiber scaffold for scarless wound healing, focusing on antimicrobial properties and tissue regeneration.
Results Summary
The optimized nanofiber mat (G14) demonstrated strong antimicrobial action against S. aureus and E. coli, enhanced wound healing in mice with dense collagen and fibroblast formation, and exhibited favorable mechanical and biocompatible properties. Analytical characterization confirmed polymer compatibility and hydrophobicity.
Population
Mice (animal model)
Effective Dosage
Not specified (different ratios of NGP and PVA were tested, with G14 being the optimized grade)
Duration
Not specified
Interactions
None mentioned
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
Neem gum polysaccharide (NGP) in conjugation with Polyvinyl alcohol (PVA) in the form of nanofibers | neutral | antimicrobial properties | - | - | exhibits | #1 |
Neem gum polysaccharide (NGP) in conjugation with Polyvinyl alcohol (PVA) in the form of nanofibers | neutral | extracellular matrix for tissue growth | - | - | mimicking | #2 |
optimized grade G14 nanofiber mats (NFM) | increase | maximum tensile strength | - | - | exhibited | #3 |
optimized grade G14 nanofiber mats (NFM) | neutral | smooth surface morphology | - | - | exhibited | #4 |
optimized grade G14 nanofiber mats (NFM) | neutral | hemocompatible properties | - | - | exhibited | #5 |
optimized grade G14 nanofiber mats (NFM) | neutral | in-vitro biodegradability | - | - | exhibited | #6 |
optimized grade G14 nanofiber mats (NFM) | neutral | antimicrobial action | S. aureus & E. coli | - | exhibited | #7 |
increased glutaraldehyde vapor for crosslinking | increase | hydrophobicity of NFM | - | - | confirmed | #8 |
G14 CL-NFM | increase | wound healing | mice | - | accelerated | #9 |
G14 CL-NFM | increase | dense collagen and fibroblasts | mice | - | resulted in | #10 |
Modern-day treatment demands scarless wound healing utilizing scaffolds in the form of nanofiber mats which are tissue and environment-friendly. Neem gum polysaccharide (NGP) in conjugation with Polyvinyl alcohol (PVA) in the form of nanofibers exhibits antimicrobial properties mimicking extracellular matrix for tissue growth. Different grades of nanofibers mats (NFM) were prepared by combining different ratios of NGP and PVA which were later crosslinked using glutaraldehyde vapors (25 % w/v in 0.5 M HCl), and optimized grade G14 exhibited maximum tensile strength with smooth surface morphology, hemocompatible properties, in-vitro biodegradability and antimicrobial action against S. aureus & E. coli. G14 was analytically characterized using different analytical techniques viz. Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), which indicated polymer-polymer compatibility. The surface hydrophobicity as detected using Optical contact angle (OCA) confirmed the hydrophobicity of NFM with increased glutaraldehyde vapor for crosslinking when compared to non-crosslinked NFM. Histopathology slides indicated G14 CL-NFM accelerated the wound healing in mice with dense collagen and fibroblasts when compared to control mice suggesting the tissue engineering potential of the prepared device.