The Protective Effects of Inulin-Type Fructans Against High-Fat/Sucrose Diet-Induced Gestational Diabetes Mice in Association With Gut Microbiota Regulation.
Study Goal
The researchers aimed to determine whether Inulin-Type Fructans (ITF) could improve glucose and lipid metabolism disorders in pregnant mice with gestational diabetes mellitus (GDM) and explore the role of gut microbiota in mediating these effects.
Results Summary
ITF treatment improved glucose and lipid metabolism, reduced fat accumulation and glucose intolerance, and altered gut microbiota composition by increasing beneficial bacteria (Verrucomicrobia, Bifidobacterium, Akkermansia) and reducing Dubosiella, which was linked to metabolic disorders.
Population
Pregnant C57BL/6J mice fed a high-fat/sucrose diet to induce GDM.
Effective Dosage
3.33 g/kg/day
Duration
Administered throughout pregnancy (exact duration not specified beyond 4 weeks pre-pregnancy and during pregnancy).
Interactions
None mentioned
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
Inulin-type fructans (ITF) | decrease | glucose and lipid metabolism disorders | C57BL/6J mice fed a high-fat/sucrose diet (HFD) to induce gestational diabetes mellitus (GDM) | - | alleviate | #1 |
ITF (3.33 g/kg/day) treatment | decrease | glucose and lipid metabolism disorder parameters | HFD-induced GDM mice | - | improved | #2 |
ITF (3.33 g/kg/day) treatment | decrease | fat accumulation | HFD-induced GDM mice | - | alleviated | #3 |
ITF (3.33 g/kg/day) treatment | decrease | glucose intolerance | HFD-induced GDM mice | - | alleviated | #4 |
ITF | increase | alpha diversity of the gut microbial community | ITF mice | - | increased | #5 |
ITF | no change | beta diversity | ITF mice | - | returned to the level of normal chow diet (NCD) mice | #6 |
inulin treatment | increase | Verrucomicrobia | - | - | obviously enriched | #7 |
inulin treatment | increase | Bifidobacterium | - | - | obviously enriched | #8 |
inulin treatment | increase | Akkermansia | - | - | obviously enriched | #9 |
inulin treatment | decrease | Dubosiella | - | - | obviously lessened | #10 |
ITF-supplemented diet | decrease | markers of glycolipid metabolism disorders | - | - | partially reversed the changes | #11 |
ITF treatment | decrease | glucose and lipid metabolism disorders | - | - | alleviate | #12 |
BACKGROUND: Inulin-type fructans (ITF) have been used as prebiotics to alleviate glucose and lipid metabolism disorders. However, few studies evaluated the microbial mechanism of ITF in improving maternal metabolic status during pregnancy. METHODS: C57BL/6J mice were fed a high-fat/sucrose diet (HFD) for 4 weeks before and throughout pregnancy to induce a model of gestational diabetes mellitus (GDM). Body weight, glycolipid metabolic parameters, and fecal short-chain fatty acids (SCFAs) were assessed in the experimental process. The effects of ITF on the fecal microbiota were analyzed by 16S rRNA gene amplicon sequencing. RESULTS: Pregnant HFD-fed mice displayed significant insulin resistance and dyslipidemia. ITF (3.33 g/kg/day) treatment improved glucose and lipid metabolism disorder parameters in HFD-induced GDM mice and alleviated fat accumulation and glucose intolerance. The alpha diversity of the gut microbial community was increased in ITF mice, while the beta diversity returned to the level of normal chow diet (NCD) mice. Interestingly, Verrucomicrobia, Bifidobacterium, and Akkermansia were obviously enriched, while Dubosiella was obviously lessened after inulin treatment. Further analysis indicated that Dubosiella was positively correlated with markers of glycolipid metabolism disorders, whereas the ITF-supplemented diet partially reversed the changes. CONCLUSION: Our results suggest that the ITF treatment may alleviate glucose and lipid metabolism disorders with the mediation of gut microbiota.