Synbiotic goat milk kefir improves health status in rats fed a high-fat and high-fructose diet.
Study Goal
The researchers aimed to determine the effects of goat milk kefir (with and without porang glucomannan) on blood glucose, HbA1c, FFAs, TNF-α, PPARg gene expression, and insulin-producing cells in rats fed a high-fat, high-fructose diet.
Results Summary
Synbiotic kefir reduced HbA1c, TNF-α, and PPARγ2 gene expression while inhibiting FFAs, but did not significantly affect plasma glucose levels or insulin-producing cells. Probiotic kefir's effects were not detailed in the abstract.
Population
Male Sprague-Dawley rats fed a high-fat, high-fructose diet.
Effective Dosage
Not specified
Duration
4 weeks
Interactions
None mentioned
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
synbiotic kefir | decrease | plasma HbA1c | rats fed HFHF diet | - | decreased | #1 |
synbiotic kefir | decrease | TNF-α | rats fed HFHF diet | - | decreased | #2 |
synbiotic kefir | decrease | FFAs | rats fed HFHF diet | - | inhibited | #3 |
synbiotic kefir | decrease | gene expression of PPARγ2 | rats fed HFHF diet | - | decreased | #4 |
synbiotic kefir | no change | total number of islets of Langerhans | rats fed HFHF diet | - | did not affect | #5 |
synbiotic kefir | no change | insulin-producing cells | rats fed HFHF diet | - | did not affect | #6 |
HFHF diet | no change | plasma glucose levels | rats | - | no significant differences | #7 |
BACKGROUND AND AIM: Kefir, a natural probiotic containing bacteria and yeast, is a fermented milk product, whereas glucomannan from porang tuber (Amorphophallus oncophyllus) is prebiotic in vivo. Simvastatin is a potent lipid-lowering statin that can be utilized for pharmacological therapy in obesity. This study aimed to determine the effect of goat milk kefir supplemented with porang glucomannan (synbiotic kefir) and goat milk kefir without glucomannan (probiotic kefir) on blood glucose, hemoglobin A1c (HbA1c), free fatty acids (FFAs), tumor necrosis factor-alpha (TNF-α), gene expression of peroxisome proliferator-activated receptor gamma (PPARg), and insulin-producing cells in rats fed a high-fat and high-fructose (HFHF) diet. MATERIALS AND METHODS: Male Sprague-Dawley rats were divided into five dietary groups: (1) Normal control, (2) rats fed HFHF, (3) rats fed HFHF+probiotic kefir, (4) rats fed HFHF+synbiotic kefir, and (5) rats fed HFHF+simvastatin. All of these treatments were administered for 4 weeks. RESULTS: There were no significant differences in plasma glucose levels in HFHF diet-fed rats before and after treatment. However, plasma HbA1c and TNF-α decreased, and FFAs were inhibited in rats after treatment with synbiotic kefir. Synbiotic kefir decreased the gene expression of PPARγ2 in HFHF diet-fed rats but did not affect the total number of islets of Langerhans and insulin-producing cells. CONCLUSION: Synbiotic kefir improved the health of rats fed an HFHF diet by decreasing HbA1c, TNF-α, and PPARγ2 gene expression and preventing an increase in FFAs.