Interaction between Flavonoids and Carotenoids on Ameliorating Oxidative Stress and Cellular Uptake in Different Cells.
Study Goal
The researchers aimed to investigate the antioxidant interactions between carotenoids (lycopene, lutein) and flavonoids (quercetin, luteolin) and their effects on cellular uptake, ROS scavenging, and antioxidant enzyme activities.
Results Summary
Combinations of carotenoids with flavonoids, particularly lycopene and quercetin at a 1:5 ratio, showed stronger antioxidant activity, enhanced cellular uptake of carotenoids, and increased expression of carotenoid transporters. Flavonoids modulated carotenoid effects on transporter expression, with synergistic groups showing improved antioxidant outcomes.
Population
HUVEC, Caco-2, and L-02 cells (in vitro study).
Effective Dosage
Total concentration of 8 μM (specific ratios varied).
Duration
Not specified.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
Flavonoids (quercetin, luteolin) and carotenoids (lycopene, lutein) combined at different molecular ratios | increase | antioxidant activity | HUVEC, Caco-2, and L-02 cells | - | showed stronger antioxidant activity | #1 |
Lycopene combined with quercetin at ratio 1:5 | increase | ROS scavenging activities | HUVEC, Caco-2, and L-02 cells | - | showed stronger ROS scavenging activities | #2 |
Lycopene combined with quercetin at ratio 1:5 | increase | Cellular antioxidant activity (CAA) units | HUVEC cells | 18 units | increased | #3 |
Lycopene combined with quercetin at ratio 1:5 | increase | Cellular antioxidant activity (CAA) units | Caco-2 cells | 12 units | increased | #4 |
Lycopene combined with quercetin at ratio 1:5 | increase | Cellular antioxidant activity (CAA) units | L-02 cells | 12 units | increased | #5 |
Lycopene combined with quercetin at ratio 1:5 | increase | SOD and CAT activities | - | - | promoted | #6 |
Flavonoids in antioxidant synergistic groups | increase | cell uptake of carotenoids | HUVEC cells | - | enhanced | #7 |
Flavonoids in antagonistic groups | decrease | cell uptake of carotenoids | HUVEC cells | - | dampened | #8 |
Synergistic group (lycopene:quercetin = 1:5) | increase | lycopene uptake | - | 271% | increased | #9 |
Antagonistic group (lutein:quercetin = 5:1) | decrease | lutein uptake | - | 17% | decreased | #10 |
Flavonoids | neutral | expression of active transporters scavenger receptor class B type I (SR-BI) or Niemann-Pick C1-like 1 (NPC1L1) | - | - | modulated the effects of carotenoids on the expression | #11 |
Synergistic group (lycopene:quercetin = 1:5) | increase | expression of SR-BI | HUVEC and Caco-2 cells | - | increased | #12 |
A diet rich in both flavonoids and lycopene | increase | antioxidant activity | - | - | possesses a great antioxidant activity | #13 |
Flavonoids (quercetin, luteolin) and carotenoids (lycopene, lutein) were combined at different molecular ratios in a total concentration of 8 μM to investigate their antioxidant interactions. Cellular uptake of carotenoids, the expression of carotenoid transporters, the ROS scavenging ability, and antioxidant enzymes activities were compared in HUVEC, Caco-2, and L-02 cells. Combinations with flavonoids in the majority showed stronger antioxidant activity. Lycopene combined with quercetin at ratio 1:5 showed stronger ROS scavenging activities, increased 18, 12, and 12 Cellular antioxidant activity (CAA) units in HUVEC, Caco-2, and L-02 cells, respectively, and promoted SOD and CAT activities than individual component. The cell uptake of carotenoids was enhanced by flavonoids in antioxidant synergistic groups, while dampened by flavonoids in antagonistic groups in HUVEC cells. The synergistic group (lycopene:quercetin = 1:5) increased lycopene uptake by 271%, while antagonistic group (lutein:quercetin = 5:1) decreased lutein uptake by 17%. Flavonoids modulated the effects of carotenoids on the expression of active transporters scavenger receptor class B type I (SR-BI) or Niemann-Pick C1-like 1 (NPC1L1). The synergistic group (lycopene:quercetin = 1:5) increased the expression of SR-BI compared to individual lycopene treatment in HUVEC and Caco-2 cells. Thus, a diet rich in both flavonoids and lycopene possesses a great antioxidant activity, especially if a higher amount of flavonoids is included.