Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) | decrease | body weights and fat mass gain upon high fat feeding in later life | mice | - | results in lower | #1 |
feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) | increase | hepatic concentrations of multiple proteins involved in β-oxidation | Male C57BL/6JOlaHsd mice | + 17% | were significantly higher | #2 |
feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) | increase | hepatic concentrations of multiple proteins involved in the TCA cycle | Male C57BL/6JOlaHsd mice | + 15% | were significantly higher | #3 |
feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) | increase | hepatic concentrations of mitochondrial antioxidative proteins | Male C57BL/6JOlaHsd mice | + 28% | were significantly higher | #4 |
feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) | increase | hepatic L-carnitine levels | Male C57BL/6JOlaHsd mice | + 33% | were higher | #5 |
feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) | increase | hepatic levels of proteins involved in fatty acid metabolism and oxidation | eIMF-fed mice | - | have higher | #6 |
feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) | no change | early life growth and body composition | Male C57BL/6JOlaHsd mice | - | were similar | #7 |
feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) | no change | cytokines | Male C57BL/6JOlaHsd mice | - | were similar | #8 |
feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) | no change | parameters of bile acid metabolism | Male C57BL/6JOlaHsd mice | - | were similar | #9 |
We recently reported that feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) results in lower body weights and fat mass gain upon high fat feeding in later life, compared to control (cIMF). To understand the underlying mechanisms, we now explored parameters possibly involved in this long-term effect. Male C57BL/6JOlaHsd mice, fed rodent diets containing eIMF or cIMF from postnatal (PN) day 16-42, were sacrificed at PN42. Hepatic proteins were measured using targeted proteomics. Lipids were assessed by LC-MS/MS (acylcarnitines) and GC-FID (fatty-acyl chain profiles). Early life growth and body composition, cytokines, and parameters of bile acid metabolism were similar between the groups. Hepatic concentrations of multiple proteins involved in β-oxidation (+ 17%) the TCA cycle (+ 15%) and mitochondrial antioxidative proteins (+ 28%) were significantly higher in eIMF versus cIMF-fed mice (p < 0.05). Hepatic L-carnitine levels, required for fatty acid uptake into the mitochondria, were higher (+ 33%, p < 0.01) in eIMF-fed mice. The present study indicates that eIMF-fed mice have higher hepatic levels of proteins involved in fatty acid metabolism and oxidation. We speculate that eIMF feeding programs the metabolic handling of dietary lipids.