The effects of spinach-derived thylakoid supplementation in combination with calorie restriction on anthropometric parameters and metabolic profiles in obese women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled clinical trial.
Study Goal
The researchers aimed to evaluate the effects of spinach-derived thylakoids combined with a calorie-restricted diet on anthropometric and metabolic profiles in obese women with PCOS.
Results Summary
The thylakoid group showed significant improvements in weight, waist circumference, fat mass, insulin levels, and insulin resistance markers compared to the placebo group. No significant differences were observed in other metabolic or hormonal parameters.
Population
Obese women with polycystic ovary syndrome (PCOS).
Effective Dosage
5 g/day thylakoid.
Duration
12 weeks.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
spinach-derived thylakoids supplementation combined with a calorie-restricted diet | decrease | weight | obese women with the polycystic ovary syndrome (PCOS) | -6.97 ± 0.52 kg vs. -3.19 ± 0.72 kg | significant decreases | #1 |
spinach-derived thylakoids supplementation combined with a calorie-restricted diet | decrease | waist circumference | obese women with the polycystic ovary syndrome (PCOS) | -7.78 ± 2.50 cm vs. -3.73 ± 1.40 cm | significant decreases | #2 |
spinach-derived thylakoids supplementation combined with a calorie-restricted diet | decrease | fat mass | obese women with the polycystic ovary syndrome (PCOS) | -5.19 ± 0.53 kg vs. -1.36 ± 0.39 kg | significant decreases | #3 |
spinach-derived thylakoids supplementation combined with a calorie-restricted diet | decrease | insulin levels | obese women with the polycystic ovary syndrome (PCOS) | -5.40 ± 1.86 vs. -1.19 ± 0.85 μU/mL | significant decreases | #4 |
spinach-derived thylakoids supplementation combined with a calorie-restricted diet | decrease | insulin resistance markers | obese women with the polycystic ovary syndrome (PCOS) | - | decreased significantly | #5 |
spinach-derived thylakoids supplementation combined with a calorie-restricted diet | decrease | serum levels of testosterone | obese women with the polycystic ovary syndrome (PCOS) | - | decreased significantly | #6 |
spinach-derived thylakoids supplementation combined with a calorie-restricted diet | no change | other parameters | obese women with the polycystic ovary syndrome (PCOS) | - | did not show significant differences | #7 |
BACKGROUND: There is a promising outlook regarding the potential effect of spinach-derived thylakoids in the management of obesity and its associated metabolic disturbances. This research aimed to evaluate the effects of spinach-derived thylakoids supplementation combined with a calorie-restricted diet on anthropometric and metabolic profiles in obese women with the polycystic ovary syndrome (PCOS). METHODS: In a 12-week double-blind placebo-controlled randomized clinical trial, 48 females with obesity and PCOS were randomly allocated into either intervention (5 g/day thylakoid) or placebo (5 g/day cornstarch) groups along with calorie-restricted diets. Anthropometric measures, physical activity levels, dietary intakes, insulin resistance markers, as well as serum levels of insulin, fasting blood glucose (FBG), non-esterified fatty acids (NEFA), and sex hormones including dehydroepiandrosterone sulfate (DHEAS), follicle-stimulating hormone (FSH), luteinizing hormone (LH), sex hormone-binding globulin (SHBG), and free androgen index (FAI) were evaluated pre-and post-intervention. RESULTS: After the 12-week intervention, there were significant decreases in weight (- 6.97 ± 0.52 vs. -3.19 ± 0.72 kg; P < 0.001), waist circumference (- 7.78 ± 2.50 vs. -3.73 ± 1.40 cm; P < 0.001), fat mass (- 5.19 ± 0.53 vs. -1.36 ± 0.39 kg; P < 0.001), and insulin levels (- 5.40 ± 1.86 vs. -1.19 ± 0.85 μU/mL; P < 0.001) in the spinach-derived thylakoid group compared to the placebo group. Furthermore, insulin resistance markers and serum levels of testosterone decreased significantly in the thylakoid group compared to the placebo group (P < 0.05). The changes in other parameters did not show significant differences between the two groups. CONCLUSIONS: Spinach-derived thylakoid supplementation resulted in more favorable improvements in anthropometric indices and insulin sensitivity compared to the calorie restriction alone. TRIAL REGISTRATION: The study was approved by the Ethics Committee of Research Vice-chancellor of Tabriz University of Medical Sciences, Tabriz, Iran, and was registered in the Iranian Registry of Clinical Trials (registration ID: IRCT20140907019082N9 ).