Dietary supplementation of olive leaf extract increases haematological, serum biochemical parameters and immune related genes expression level in common carp (Cyprinus carpio) juveniles.
Study Goal
The researchers aimed to determine the effects of dietary olive leaf extract (OLE) on blood parameters, immune-related gene expression, and resistance to Edwardsiella tarda in common carp.
Results Summary
OLE supplementation improved hematocrit ratio, serum myeloperoxidase activity, immune response gene levels (IL-1β in head kidney and TNF-α in spleen), and survival rate against E. tarda, with the 0.1% OLE group showing notable benefits. The study concluded that 1 g/kg OLE over 60 days effectively enhanced immune parameters and disease resistance in common carp.
Population
Common carp (Cyprinus carpio) with a mean body weight of 15.90 ± 0.93 g.
Effective Dosage
0.0%, 0.1%, 0.25%, 0.50%, and 1.0% OLE in diet.
Duration
60 days.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
dietary olive leaf (Olea europea L.) extract (OLE) | increase | haematocrit ratio | common carp, Cyprinus carpio | - | Increased | #1 |
dietary olive leaf (Olea europea L.) extract (OLE) | increase | serum myeloperoxidase activity | common carp, Cyprinus carpio | - | Increased | #2 |
dietary olive leaf (Olea europea L.) extract (OLE) | increase | immune response gene levels (IL-1β in head kidney tissue) | common carp, Cyprinus carpio | - | Increased | #3 |
dietary olive leaf (Olea europea L.) extract (OLE) | increase | immune response gene levels (TNF-α in spleen tissue) | common carp, Cyprinus carpio | - | Increased | #4 |
dietary olive leaf (Olea europea L.) extract (OLE) | increase | survival rate against E. tarda | common carp, Cyprinus carpio | - | Increased | #5 |
dietary olive leaf (Olea europea L.) extract (OLE) at 0.1% | increase | survival rate against E. tarda | common carp, Cyprinus carpio | - | Increased | #6 |
feeding common carp with a diet containing 1 g/kg OLE over a period of 60 days | increase | fish immune parameters | common carp | - | improve | #7 |
feeding common carp with a diet containing 1 g/kg OLE over a period of 60 days | increase | survival rate against E. tarda | common carp | - | improve | #8 |
The present study investigated the effects of dietary olive leaf (Olea europea L.) extract (OLE) on some blood parameters and immune (TNF-α, IL-1ß and IL-8) related genes in different tissues (head kidney, liver and spleen) and resistance of common carp, Cyprinus carpio to Edwardsiella tarda. Five diets were prepared for fed fish (mean body weight 15.90 ± 0.93 g) with different rates of OLE (0.0%, 0.1%, 0.25%, 0.50% and 1.0%). A control diet was prepared non-supplemented with OLE. Increased haematocrit ratio, serum myeloperoxidase activity, immune response gene levels (IL-1β in head kidney tissue and TNF-α in spleen tissue) and survival rate against E. tarda particularly in the 0.1% OLE treatment group. In conclusion, results of the present study show that feeding common carp with a diet containing 1 g/kg OLE over a period of 60 days might be adequate to improve fish immune parameters, and survival rate against E. tarda. Therefore, OLE can be used as a dietary additive to prevent E. tarda in common carp.