Caralluma fimbriata and metformin protection of rat pancreas from high fat diet induced oxidative stress.
Study Goal
The researchers aimed to determine whether Caralluma fimbriata extract (CFE) could protect against high-fat-diet-induced oxidative stress and pancreatic damage in rats, comparing its effects to metformin.
Results Summary
CFE prevented oxidative stress markers (reduced lipid/protein oxidation, increased glutathione, normalized antioxidant enzyme activity) and histological damage (acinar cell degeneration, necrosis, edema, hemorrhage) in high-fat-diet-fed rats, with efficacy comparable to metformin. No major limitations were noted, but human applicability remains unconfirmed.
Population
Male Wistar rats fed a high-fat diet (no human data).
Effective Dosage
Not specified (oral administration for 90 days).
Duration
90 days.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
high fat diet | increase | oxidative stress | - | - | promotes | #1 |
high fat diet | increase | pancreatic fibrosis | - | - | contributes to the development of | #2 |
high fat diet | increase | lipid oxidation (LO) | Wistar rats | - | elevated | #3 |
high fat diet | increase | protein oxidation (PO) | Wistar rats | - | elevated | #4 |
high fat diet | decrease | reduced glutathione (GSH) | Wistar rats | - | decreased | #5 |
high fat diet | decrease | antioxidants | Wistar rats | - | decreased activities of | #6 |
high fat diet | increase | polyol pathway enzymes, aldose reductase (AR) and sorbitol dehydrogenase (SDH) | Wistar rats | - | increased activities of | #7 |
hydroalcoholic extract of Caralluma fimbriata (CFE) | decrease | oxidative stress | Wistar rats fed a high fat diet | - | prevented | #8 |
metformin (Met) | decrease | oxidative stress | Wistar rats fed a high fat diet | - | prevented | #9 |
hydroalcoholic extract of Caralluma fimbriata (CFE) | increase | antioxidant status | control group rats | - | exhibited improved | #10 |
hydroalcoholic extract of Caralluma fimbriata (CFE) | decrease | high fat diet induced acinar cell degeneration, necrosis, edema and hemorrhage | Wistar rats | - | prevented | #11 |
A high fat diet promotes oxidative stress, which contributes to the development of pancreatic fibrosis. We compared the protective effects of a hydroalcoholic extract of Caralluma fimbriata (CFE) to metformin (Met) in the pancreas of Wistar rats fed a high fat diet. The experimental animals were divided into five groups: control (C), treated with CFE (C + CFE), treated with high fat diet (HFD), high fat diet treated with CFE (HFD + CFE), and high fat diet treated with metformin (Met) (HFD + Met). CFE was administered orally to groups C + CFE and HFD + CFE rats for 90 days. Met was given to the HFD + Met group. After 90 days, oxidative stress markers in the pancreas including reduced glutathione (GSH), lipid oxidation (LO), protein oxidation (PO), and activities of antioxidant and polyol pathway enzymes, aldose reductase (AR) and sorbitol dehydrogenase (SDH) were assayed and tissue histology was examined. Establishment of oxidative stress in high fat diet fed rats was verified by elevated LO and PO, decreased GSH, decreased activities of antioxidants and increased activities of polyol pathway enzymes. Oxidative stress was prevented in HFD + CFE and HFD + Met groups. Group C + CFE exhibited improved antioxidant status compared to group C. CFE treatment prevented high fat diet induced acinar cell degeneration, necrosis, edema and hemorrhage. CFE could be used as adjuvant therapy for preventing or managing high fat diet induced pancreatic damage.