Amla (Emblica officinalis) improves hepatic and renal oxidative stress and the inflammatory response in hypothyroid female wistar rats fed with a high-fat diet.
Study Goal
The researchers aimed to determine whether amla (Emblica officinalis) extract could mitigate oxidative stress and inflammatory response in hypothyroid rats fed a high-fat diet, as a model for hypothyroidism with obesity.
Results Summary
Amla extract significantly restored redox balance and reduced inflammatory signaling in hypothyroid rats with diet-induced obesity, countering the synergistic worsening of oxidative stress and inflammation caused by hypothyroidism and high-fat diet. No adverse effects were reported, but the study was limited to an animal model.
Population
Female Wistar rats (5-months-old) with induced hypothyroidism and obesity via propylthiouracil (PTU) and high-fat diet (HFD).
Effective Dosage
100 mg/kg body weight/day of amla extract.
Duration
6 weeks.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
Propylthiouracil (PTU) | increase | oxidative stress and inflammatory response | rats | - | caused | #1 |
Propylthiouracil (PTU) | increase | plasma MDA, TNF-α, CRP and GPx | rats | - | increased | #2 |
Propylthiouracil (PTU) | decrease | levels of TAS and reduced glutathione (GSH) | rats | - | decreased | #3 |
Propylthiouracil (PTU) | increase | expressions of pERK, pP38, TNF-α, IL6, COX2 and NOX-4 | rats | - | up-regulated | #4 |
high-fat diet (HFD) | increase | oxidative stress and inflammatory response | rats | - | caused | #5 |
high-fat diet (HFD) | increase | plasma MDA, TNF-α, CRP and GPx | rats | - | increased | #6 |
high-fat diet (HFD) | decrease | levels of TAS and reduced glutathione (GSH) | rats | - | decreased | #7 |
high-fat diet (HFD) | increase | expressions of pERK, pP38, TNF-α, IL6, COX2 and NOX-4 | rats | - | up-regulated | #8 |
hypothyroidism (HT) followed by high-fat diet (HFD) | increase | all these metabolic derangements | rats | - | augmented | #9 |
amla fruit extract | decrease | the redox imbalance and inflammatory signaling | hypothyroid rats fed with a high-fat diet | - | significantly restored | #10 |
amla fruit extract | decrease | oxidative stress and inflammatory response | hypothyroid rats fed with a high-fat diet | - | ameliorated | #11 |
BACKGROUND: We investigated the protective effects of amla (Emblica officinalis) on the pathogenesis of oxidative stress (OS) and inflammatory response in hypothyroid rats fed with a high-fat diet (HFD) as an experimental model of hypothyroidism (HT) with obesity. METHODS: A total of 80 female wistar rats (5-months-old) were divided into eight different groups. Propylthiouracil (PTU) and HFD were used to induce the experimental HT and obesity, respectively. The euthyroid and hypothyroid rats were fed either normal chow or HFD with and without amla extract (AE, 100 mg/kg bw/day) for 6 weeks. The blood and tissues, liver and kidney OS and inflammatory parameters were studied using appropriate biochemical and molecular techniques. RESULTS: PTU and HFD per se caused OS and inflammatory response as evidenced by increased plasma MDA, TNF-α, CRP and GPx in association with decreased levels of TAS and reduced glutathione (GSH). The proteomic analysis revealed that the expressions of pERK, pP38, TNF-α, IL6, COX2 and NOX-4 were up-regulated in the liver and kidney of these rats. In addition, all these metabolic derangements were further augmented when HT was followed by the addition of HFD. This suggested that there was a synergism between HT and the intake of HFD on the development of OS and inflammatory response. CONCLUSIONS: The treatment with amla fruit extract significantly restored the redox imbalance and inflammatory signaling and ameliorated OS and inflammatory response, suggesting the use of this natural compound as an alternative remedy or adjuvant for the management of metabolic complications concomitant with HT.