Renoprotective effect of Bacopa monnieri via inhibition of advanced glycation end products and oxidative stress in STZ-nicotinamide-induced diabetic nephropathy.
Study Goal
The researchers aimed to evaluate the nephroprotective effects of Bacopa monnieri extracts and stigmasterol in diabetic nephropathy using a rat model.
Results Summary
The study found that Bacopa monnieri extracts and stigmasterol significantly reduced serum glucose, uric acid, creatinine, and lipid levels, improved antioxidant markers (SOD, GSH), and decreased lipid peroxidation and AGE formation in kidneys.
Population
Male Wistar rats with streptozotocin-induced diabetes.
Effective Dosage
Extracts (100, 200, 400 mg/kg), stigmasterol (5, 10 mg/kg).
Duration
45 days.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
alcohol and hydroalcohol extract of Bacopa monnieri | decrease | serum glucose level | male wistar rats with streptozotocin-induced diabetes | - | produced significant attenuation | #1 |
alcohol and hydroalcohol extract of Bacopa monnieri | decrease | uric acid | male wistar rats with streptozotocin-induced diabetes | - | produced significant attenuation | #2 |
alcohol and hydroalcohol extract of Bacopa monnieri | decrease | creatinine | male wistar rats with streptozotocin-induced diabetes | - | produced significant attenuation | #3 |
alcohol and hydroalcohol extract of Bacopa monnieri | decrease | lipid levels | male wistar rats with streptozotocin-induced diabetes | - | produced significant attenuation | #4 |
alcohol and hydroalcohol extract of Bacopa monnieri | increase | superoxide dismutase (SOD) | male wistar rats with streptozotocin-induced diabetes | - | improvement in the level | #5 |
alcohol and hydroalcohol extract of Bacopa monnieri | increase | glutathione (GSH) | male wistar rats with streptozotocin-induced diabetes | - | improvement in the level | #6 |
alcohol and hydroalcohol extract of Bacopa monnieri | decrease | lipid peroxidation in terms of TBARS | male wistar rats with streptozotocin-induced diabetes | - | decrease | #7 |
alcohol and hydroalcohol extract of Bacopa monnieri | decrease | formation of AGEs in kidneys | male wistar rats with streptozotocin-induced diabetes | - | significantly reduced | #8 |
stigmasterol isolated from B. monnieri | decrease | serum glucose level | male wistar rats with streptozotocin-induced diabetes | - | produced significant attenuation | #9 |
stigmasterol isolated from B. monnieri | decrease | uric acid | male wistar rats with streptozotocin-induced diabetes | - | produced significant attenuation | #10 |
stigmasterol isolated from B. monnieri | decrease | creatinine | male wistar rats with streptozotocin-induced diabetes | - | produced significant attenuation | #11 |
stigmasterol isolated from B. monnieri | decrease | lipid levels | male wistar rats with streptozotocin-induced diabetes | - | produced significant attenuation | #12 |
stigmasterol isolated from B. monnieri | increase | superoxide dismutase (SOD) | male wistar rats with streptozotocin-induced diabetes | - | improvement in the level | #13 |
stigmasterol isolated from B. monnieri | increase | glutathione (GSH) | male wistar rats with streptozotocin-induced diabetes | - | improvement in the level | #14 |
stigmasterol isolated from B. monnieri | decrease | lipid peroxidation in terms of TBARS | male wistar rats with streptozotocin-induced diabetes | - | decrease | #15 |
stigmasterol isolated from B. monnieri | decrease | formation of AGEs in kidneys | male wistar rats with streptozotocin-induced diabetes | - | significantly reduced | #16 |
Hyperglycemia and oxidative stress are involved in the development of diabetic nephropathy (DN). This study was designed to evaluate the effect of alcohol and hydroalcohol extract of Bacopa monnieri and stigmasterol isolated from B. monnieri in the treatment of DN. Diabetes was induced in male wistar rats by streptozotocin (65 mg/kg i.p.) 15 min after nicotinamide (230 mg/kg, i.p.) administration. After 30 days, the rats were treated with different doses of extracts (100, 200, and 400 mg/kg) and stigmasterol (5 and 10 mg/kg) for 45 days to analyze their nephroprotective effect and produced significant attenuation in the serum glucose level, uric acid, creatinine, and lipid levels. Moreover, there is improvement in the level of superoxide dismutase (SOD), glutathione (GSH), and decrease in lipid peroxidation in terms of TBARS. The formation of AGEs in kidneys was also significantly reduced. These findings suggest that B. monnieri and its isolate (stigmasterol) might inhibit the progression of DN.