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High-Protein Intake during Weight Loss Therapy Eliminates the Weight-Loss-Induced Improvement in Insulin Action in Obese Postmenopausal Women.

Cell reports
January 1, 1970
Gordon I Smith et al. (8 authors)
Clinical TrialJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tHuman StudyClinical
Study Details

Study Goal

The researchers aimed to compare the effects of a standard-protein (0.8 g/kg/day) versus a high-protein (1.2 g/kg/day) hypocaloric diet on muscle insulin action and lean tissue mass preservation in postmenopausal women with obesity during weight loss.

Results Summary

The high-protein diet reduced lean tissue mass loss by ~45% but prevented weight-loss-induced improvements in muscle insulin signaling, glucose uptake, and adaptations in oxidative stress and cell structural biology pathways.

Population

Postmenopausal women with obesity.

Effective Dosage

0.8 g protein/kg/day (standard-protein) vs. 1.2 g protein/kg/day (high-protein).

Duration

Not specified in the abstract.

Interactions

None mentioned.

Extracted Claims (7)
InterventionDirectionEndpointPopulationDosageImpactClaim #
High-protein (HP) intake during weight loss (WL) therapy
decrease
lean tissue mass
-
-
reduces the loss of
#1
protein ingestion
decrease
postprandial insulin sensitivity
-
-
reduces
#2
hypocaloric diet containing 1.2 g protein/kg/day
decrease
lean tissue mass
postmenopausal women with obesity
∼45%
reduced the WL-induced decline in
#3
hypocaloric diet containing 1.2 g protein/kg/day
no change
muscle insulin signaling
postmenopausal women with obesity
-
prevented the WL-induced improvements in
#4
hypocaloric diet containing 1.2 g protein/kg/day
no change
insulin-stimulated glucose uptake
postmenopausal women with obesity
-
prevented the WL-induced improvements in
#5
hypocaloric diet containing 1.2 g protein/kg/day
no change
oxidative stress pathways
postmenopausal women with obesity
-
prevented the WL-induced adaptations in
#6
hypocaloric diet containing 1.2 g protein/kg/day
no change
cell structural biology pathways
postmenopausal women with obesity
-
prevented the WL-induced adaptations in
#7
Abstract

High-protein (HP) intake during weight loss (WL) therapy is often recommended because it reduces the loss of lean tissue mass. However, HP intake could have adverse effects on metabolic function, because protein ingestion reduces postprandial insulin sensitivity. In this study, we compared the effects of ∼10% WL with a hypocaloric diet containing 0.8 g protein/kg/day and a hypocaloric diet containing 1.2 g protein/kg/day on muscle insulin action in postmenopausal women with obesity. We found that HP intake reduced the WL-induced decline in lean tissue mass by ∼45%. However, HP intake also prevented the WL-induced improvements in muscle insulin signaling and insulin-stimulated glucose uptake, as well as the WL-induced adaptations in oxidative stress and cell structural biology pathways. Our data demonstrate that the protein content of a WL diet can have profound effects on metabolic function and underscore the importance of considering dietary macronutrient composition during WL therapy for people with obesity.

Medical Subject Headings (MeSH)
BiomarkersBlood GlucoseDiet, ReducingDietary ProteinsFatty AcidsFemaleGene Expression ProfilingGene Expression RegulationHormonesHumansInflammationInsulinKineticsLipogenesisMetabolomeMiddle AgedMitochondriaMusclesObesityOxidation-ReductionOxidative StressPalmitic AcidPostmenopauseWeight Loss
Study Links
Quality Scores
Safety60
Efficacy70/10
Quality80/10
Citation Metrics
Total Citations87
Citations/Year9.7
Relative Citation Ratio3.34
NIH Percentile87.1%
Research Impact Scores
APT Score0.95
Weight Score1.93
Normalized Score0.68
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