Effects of probiotic yogurt on fat distribution and gene expression of proinflammatory factors in peripheral blood mononuclear cells in overweight and obese people with or without weight-loss diet.
Study Goal
The researchers aimed to determine if probiotic yogurt containing Lactobacillus casei DN001, combined with a weight-loss diet, could reduce proinflammatory markers and improve metabolic outcomes in overweight and obese individuals.
Results Summary
The study found that probiotic yogurt with a low-calorie diet (PLCD) led to significant reductions in BMI, fat percentage, leptin levels, and ROR-γt gene expression compared to other groups. However, TNF-α expression remained unchanged, and hs-CRP reduction was more evident in the group consuming probiotic yogurt without a diet (PWLCD).
Population
Healthy overweight and obese individuals (n = 75).
Effective Dosage
200 g/day of yogurt containing Lactobacillus casei DN001 (10^8 CFU/g), along with Lactobacillus acidophilus La5 and Bifidobacterium BB12.
Duration
8 weeks.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
probiotic yogurt with low-calorie diet (PLCD) | decrease | body mass index (BMI) | overweight and obese individuals | - | reduction was more obvious | #1 |
probiotic yogurt with low-calorie diet (PLCD) | decrease | fat percentage | overweight and obese individuals | - | reduction was more obvious | #2 |
probiotic yogurt with low-calorie diet (PLCD) | decrease | leptin level | overweight and obese individuals | - | reduction was more obvious | #3 |
probiotic yogurt with low-calorie diet (PLCD) | decrease | gene expression of ROR-γt | overweight and obese individuals | - | reduction was significant | #4 |
probiotic yogurt with low-calorie diet (PLCD) | no change | expression of TNF-α | overweight and obese individuals | - | did not change | #5 |
regular yogurt with low-calorie diet (RLCD) | no change | expression of TNF-α | overweight and obese individuals | - | did not change | #6 |
probiotic yogurt without low-calorie diet (PWLCD) | no change | expression of TNF-α | overweight and obese individuals | - | did not change | #7 |
probiotic yogurt with low-calorie diet (PLCD) | decrease | mean concentration of leptin | overweight and obese individuals | -2.38 ng/mL | significantly decreased | #8 |
regular yogurt with low-calorie diet (RLCD) | decrease | mean concentration of leptin | overweight and obese individuals | -1.75 ng/mL | significantly decreased | #9 |
probiotic yogurt without low-calorie diet (PWLCD) | decrease | mean concentration of leptin | overweight and obese individuals | -0.55 ng/mL | decreased | #10 |
probiotic yogurt without low-calorie diet (PWLCD) | decrease | serum levels of hs-CRP | overweight and obese individuals | -3.4 pg/mL | reduction was more evident | #11 |
probiotic yogurt with low-calorie diet (PLCD) | decrease | serum levels of hs-CRP | overweight and obese individuals | -1.76 pg/mL | reduction | #12 |
regular yogurt with low-calorie diet (RLCD) | decrease | serum levels of hs-CRP | overweight and obese individuals | -2.98 pg/mL | reduction | #13 |
weight-loss diet and probiotic yogurt | neutral | T-cells subset specific gene expression in PBMCs | overweight and obese individuals | - | had synergistic effects | #14 |
weight-loss diet and probiotic yogurt | neutral | fat percentage | overweight and obese individuals | - | had synergistic effects | #15 |
weight-loss diet and probiotic yogurt | neutral | body weight | overweight and obese individuals | - | had synergistic effects | #16 |
OBJECTIVES: The purpose of this study was to investigate whether probiotics had an effect on proinflammatory markers and cytokines in overweight and obese individuals and whether they could have synergistic effects with weight-loss diets. METHODS: A total of 75 healthy overweight and obese individuals completed this randomized doubled-blind controlled clinical trial. Participants were randomly assigned to groups consuming regular yogurt with a low-calorie diet (LCD, RLCD; n = 25) or receiving probiotic yogurt with LCD (PLCD; n = 25) or consuming probiotic yogurt without LCD (PWLCD; n = 25) for 8 weeks. The pribiotic regimen contained 200 g/day yogurt, enriched by Lactobacillus acidophilus La5, Bifidobacterium BB12, and Lactobacillus casei DN001 10(8) colony-forming units/g. Body fat percentage, high-sensitive C-reactive protein (hs-CRP), tumor necrosis factor-alpha (TNF-α), leptin, and mRNA levels of inflammation-related genes (TNF-α and RAR-related orphan receptor gamma [ROR-γt]) in peripheral blood mononuclear cells (PBMCs) were measured. RESULTS: A reduction in body mass index (BMI), fat percentage, and leptin level was observed that was more obvious in groups who received the weight-loss diet with probiotic yogurt. Reduction in the gene expression of ROR-γt was significant in the PLCD group (p < 0.001). The expression of TNF-α did not change among all groups after intervention. The mean concentration of leptin was significantly decreased in all groups after the dietary intervention, but the mean changes in leptin level in the PLCD group was more prominent compared to the other two groups (-2.38, p < 0.001 [PLCD] vs -1.75, p = 0.002 [RLCD] and -0.55 ng/mL, p = 0.12 [PWLCD]). The reduction in serum levels of hs-CRP was more evident in the PWLCD group compared to the PLCD and RLCD groups after the 8-week intervention (-3.4, p = 0.03 vs -1.76, p < 0.001 and -2.98 pg/mL, p < 0.001, respectively). CONCLUSION: Our results suggested that the weight-loss diet and probiotic yogurt had synergistic effects on T-cells subset specific gene expression in PBMCs, fat percentage, and body weight among overweight and obese individuals.